تگ پلیمراز (Taq polymerase) یک DNA پلیمراز مقاوم به حرارت است که اولین بار در سال ۱۹۷۶ توسط Chien و همکارانش از یک باکتری گرما دوست بنام Thermus aquaticus استخراج شد. این آنزیم به وفور در واکنش زنجیره ای پلیمراز (PCR) مورد استفاده قرار می‌گیرد.
ورود به صفحه خرید Taq DNA Polymerase
باکتری Thermus aquaticus از چشمه‌های آب گرم استخراج شده‌است. این باکتری دارای آنزیم تگ پلیمراز است. این آنزیم توانایی تحمل دمای بالا را دارد و به همین دلیل در تکنیک PCR کاربرد فراوانی یافته است.
دمای بهینه برای فعالیت آنزیم تگ پلیمراز، ۷۵ تا ۸۰ درجه سانتیگراد است. این آنزیم در دمای ۷۲ درجه سانتیگراد قادر است ۱۰۰۰ جفت باز را در کمتر از ۱۰ ثانیه رونویسی کند.
یکی از نقاط ضعف این آنزیم عدم توانایی آن برای فعالیت تحصیح اگزونوکلئازی (exonuclease proofreading) در جهت ′3 به ′5 است که این باعث می‌شود دقت کار پایین بیاید. هم چنین منجر به موتاسیون شده و اختصاصیت آن کم است.
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рџ“‹ Your United XI to face Middlesbrough this evening 🔴#MUFC | #FACup Rangers duo James Sands and Malik Tillman have been overlooked for next month's World Cup in Qatar, but it's better news for Celtic's Cameron Carter-Vickers who will be on the plane. WOULD YOU BELIEVE IT рџ±@mdrcrooks scores against the club he supports for @Boro! рџ’Ґ#EmiratesFACup pic.twitter.com/dgtNknW8c3 LEAGUES | LIVESCORE | GAMES | TEAMS | STANDINGS | STATISTICS | BOOKMAKERS | ODDS The 2023 IHF Men’s World Championship is just around the corner. We will throw off the coverage of the competition by taking a comprehensive look at the eight preliminary round groups, analysing the teams and pointing out the patterns in their head-to-head records to get a glimpse of what to expect…
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This study suggests some important points not only about the emergency treatment of hypercalcemia, but also about how we formulate and review therapies in general
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