کیت استخراج DNA از مدفوع |Stool DNA isolation kit

خانه/فروشگاه/کیت آزمایشگاهی/کیت استخراج DNA/کیت استخراج DNA از مدفوع |Stool DNA isolation kit

کیت استخراج DNA از مدفوع |Stool DNA isolation kit

کیت استخراج DNA از مدفوع |Stool DNA isolation kit

برند: دسته: کیت آزمایشگاهی, کیت استخراج DNA

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کیت استخراج DNA از مدفوع |Stool DNA isolation kit

25Prep: FPKT001.0025

50Prep: FPKT001.0050

100Prep: FPKT001.0100

از: 10,500,000 ریال

تعداد Preparation را انتخاب کنید
ضروری
100 50 25
پاک کردن انتخاب‌ها

از: 10,500,000 ریال

تحویل اکسپرس
پشتیبانی 24 ساعته
پرداخت در محل
7 روز ضمانت بازگشت
ضمانت اصل بودن کالا
لوگو برند کیاژن

توضیحات

کیت استخراج DNA از مدفوع |Stool DNA isolation kit

STDNA

Stool DNA Isolation Kit

Cat. No:

FPKT001.0025

FPKT001.0050

FPKT001.0100

Contents:

Stool Dna Extraction kit

Kit storage:

This kit should be stored at room temperature.

Proteinase K, Lysozyme and RNase A should be stored at -20 °C

If properly stored, all kit components are stable until the expiration date printed on the label.

After adding RNase A to DG1 buffer and absolute ethanol to wash buffers, Store the DG1 and wash Buffers at +2 to +8°C

Additional Equipment and Reagent required

  • Absolute ethanol
  • Standard tabletop microcentrifuge capable of 13,000 x g centrifugal force
  • Microcentrifuge tubes, 1.5 ml, sterile

Application

This kit provides a convenient and rapid method to isolate total DNA from fresh, frozen and preserved stool samples. The purified DNA is of the highest quality and is fully compatible with all downstream applications such as PCR, qPCR, NGS and microarrays since all humic acid substances and other PCR inhibitors are removed during the isolation process.

The procedure is optimized to achieve reliable results within 90 min.

Handling Requirements and Safety Information

All solutions are clear and should not be used when precipitates have formed. Warm the solutions at +15 to +25°C or in a 37°C water bath until the precipitates have dissolved.

DG3 Buffer contain guanidinium hydrochloride which is an irritant.

Do not allow DG2 Buffer and DG3 Buffer to touch your skin, eyes, or mucous membranes. If contact does occur, wash the affected area immediately with large amounts of water. 

If you spill the reagent, dilute the spill with water before wiping it up.

Do not use any modified ethanol.

Do not pool reagents from different lot numbers.

Immediately after usage, close all bottles in order to avoid leakage, varying buffer concentrations or buffer conditions.

After first opening store all bottles in an upright position.

Do not allow the DG2 Buffer and DG3 Buffer to mix with sodium hypochlorite found in commercial bleach solutions. This mixture can produce a highly toxic gas.

Wear protective disposable gloves, laboratory coats and eye protection, when handling samples and kit reagents.

Do not contaminate the reagents with bacteria, virus, or nucleases. Use disposable pipets and nuclease free pipet tips only, to remove aliquots from reagent bottles.

preparation procedure:

Working Solution Preparation

Add RNase A to DG1 Buffer completely Dissolve the RNase A by vortexing. store the DG1 buffer at 4 °C.

FPKT001.0025-25 preps

Add 16 ml absolute ethanol to Wash Buffer Bottle.

FPKT001.0050-50 preps

Add 32 ml absolute ethanol to Wash Buffer Bottle.

FPKT001.0100-100 preps

Add 64 ml absolute ethanol to Wash Buffer Bottle.

Please note that the Ethanol concentration of a Washing Buffer may decrease during long term storage resulting in a drop-down of the final DNA yield.

before starting Incubate the Elution buffer at 55 to 65 ° C until the end of the protocol to obtain the maximum yields.

Protocol

  1. Sample preparation

Add one bead to a 2ml microtube. Add up to 200 mg of stool sample to a bead tube and place the tube on ice.

If the sample is dry, reduce the sample size to ≤ 50 mg.

If the sample is liquid, add 200 μl of sample into a bead tube.

  1. Add 200 μl DG1 Buffer to the 2ml microtube. Vortex at maximum speed for 5 minutes. Incubate the sample mixture at 60 °C for 20 minutes and vortex the sample for every 5 minutes during the incubation.

Make sure stool sample is homogenized completely.

  1. Add 15 μl Lysozyme to the sample microtube and incubate at 37° C for 30 minutes. vortex the sample for every 5 minutes during the incubation.
  1. Add 200 μl DG2 Buffer to the microtube and mix them by Vortex.
  1. Add 2.5 μl of Proteinase K then mix by vortex. Incubate at 60 °C for at least 15 minutes. During incubation, invert the tube every 3 minutes.
  1. Add 400 μl of DG3 Buffer to the sample and mix by vortex for 10 seconds. Briefly spin the tube to remove drops from the inside of the lid.
  1. Incubate the sample mixture on ice for 5 minutes Centrifuge at full speed (~18,000 x g) for 5 minutes.
  1. Transfer the supernatant into a DNA spin column assembled in a clean collection tube (provided). Centrifuge at 10000 g for 1 min at room temperature.
  1. Disconnect the DNA spin column from collection tube and discard the flow through solution. Reconnect the DNA spin column to the collection tube.
  1. Add 500 μl Wash Buffer to DNA spin column and centrifuge at 10000 g for 1 min. Discard flow through.
  1. Centrifuge the DNA spin column at 10000 g for 1 min to remove residual ethanol.
  1. Place the DNA spin column into the clean new microtube. Add 30 μl of pre-warmed Elution Buffer or sterile DDW directly onto column membrane and stand for 3 min. Centrifuge at 10000 g for 1 min.
  2. Add another 30 μl pre-warmed Elution Buffer or sterile DDW directly onto column membrane and stand for 3 min. Centrifuge at 10000 g for 1 min.
  3. Check 7-10 μl of extracted DNA by 1% agarose gel electrophoresis. Store DNA at -20 ̊C

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