Choose the High Pure Viral Nucleic Acid Kit to efficiently isolate viral DNA and RNA from a broad range of research sample materials, including:
Serum, plasma, whole blood, cell culture supernatant, peripheral blood mono nuclear cells (PBMCs), cerebrospinal fluid (CSF), tongue scrapings, and throat wash samples.

Use a simple, rapid protocol to generate high-quality templates for direct use in PCR, RT-PCR, qPCR, and qRT-PCR applications.

 Use one versatile kit for diverse applications.

Isolate viral DNA and RNA from a wide variety of sample materials, enabling simultaneous analysis of both virus types.

 Save time with a rapid, easy-to-use protocol.

Prepare multiple PCR/RT-PCR templates in 20 minutes, with only 10 minutes of hands-on time.

 Maximize performance and accuracy in downstream assays.

Obtain highly pure, concentrated (50 μl) nucleic acids that provide high sensitivity, reproducibility, and specificity in real-time PCR and other applications.

Protocol for isolating viral nucleic acids from 200 μl serum, plasma, or whole blood.

For the isolation of nucleic acids from whole blood, pre-warm the elution buffer to +70°C.

1-To a nuclease-free 1.5 ml microcentrifuge tube:

• Add 200 µl serum, plasma, or whole blood.
• Add 200 µl working solution, freshly prepared (carrier RNA-supplemented Binding Buffer).
• Add 50 µl Proteinase K solution; mix immediately.
• Incubate for 10 min at +72°C.

2-Add 100 µl Binding Buffer and mix well.

3-To transfer the sample to a High Pure Filter Tube:
• Insert one High Pure Filter Tube into one Collection Tube.
• Pipet the entire sample into the upper reservoir of the Filter Tube.

4-Insert the entire High Pure Filter Tube assembly into a standard table-top centrifuge.

• Centrifuge 1 min at 8,000 × g.

5-After centrifugation:

• Remove the Filter Tube from the Collection Tube; discard the flow through and the Collection Tube.
• Combine the Filter Tube with a new Collection Tube.

6-After combining the Filter Tube with a new Collection Tube:

• Add 500 µl Inhibitor Removal Buffer to the upper reservoir of the Filter Tube.
• Centrifuge 1 min at 8,000 × g.

7-After centrifugation:

• Remove the Filter Tube from the Collection Tube; discard the flow through and the Collection Tube.
• Combine the Filter Tube with a new Collection Tube.

8-After removal of inhibitors:
• Add 450 µl Wash Buffer to the upper reservoir of the Filter Tube. • Centrifuge 1 min at 8,000 × g and discard the flow through.

9-After the first wash and centrifugation:

• Remove the Filter Tube from the Collection Tube; discard the flow through and the Collection Tube.
• Combine the Filter Tube with a new Collection Tube.
• Add 450 µl Wash Buffer to the upper reservoir of the Filter Tube.
• Centrifuge 1 min at 8,000 × g and discard the flow through.
• Leave the Filter Tube-Collection Tube assembly in the centrifuge and spin it for 10 s at maximum speed (approx. 13,000 × g) to remove any residual Wash Buffer. The extra centrifugation time ensures removal of residual Wash Buffer.

10-Discard the Collection Tube and insert the Filter Tube into a nuclease- free, sterile 1.5 ml microcentrifuge tube.

11-To elute the viral nucleic acids:
• Add 50 µl Elution Buffer to the upper reservoir of the Filter Tube. • Centrifuge the tube assembly for 1 min at 8,000 × g.

12-The microcentrifuge tube contains the eluted, purified viral nucleic acids.

Use the eluted nucleic acids directly in PCR (10 – 20 µl DNA eluate) or RT-PCR (3.5 µl viral RNA); or, store the eluted viral RNA at –80°C or the viral DNA at +2 to +8°C or –15 to –25°C for later analysis.