آنزیم RsaI (10 U/µL) | کمپانی Thermo Fisher Scientific

آنزیم RsaI (10 U/µL) | کمپانی Thermo Fisher Scientific

Catalog Number: ER1121

Unit Size: 1000 Units

Catalog Number: ER1122

Unit Size: 5000 Units

5′			G	T ↓	A	C		3′
3′			C	A ↑	T	G		5′

Thermo Scientific RsaI restriction enzyme recognizes GT^AC sites and cuts best at 37°C in Tango buffer. See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Note: Also available as a FastDigest enzyme for rapid DNA digestion. Isoschizomers: AfaI, CviQI, RsaNI.

Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

Features

• Superior quality—stringent quality control and industry leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
• Wide selection of restriction endonuclease specificities

Applications

• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern blotting
• Restriction fragment length polymorphism (RFLP)
• SNP

Note: For methylation sensitivity, refer to product specifications.

	Specifications
10U/μL	Concentration
CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive	Methylation Sensitivity
Rsa I	Enzyme
10X Buffer Tango	Compatible Buffer
Yes	Sensitive to Heat Inactivation
37°C	Optimal Reaction Temperature
No	Type IIS RE
5000U	Quantity
Restriction Enzyme	Product Type
Traditional cloning	Research Category

آنزیم RsaI (10 U/µL) | کمپانی Thermo Fisher Scientific