آنزیم Eco47III (AfeI) (10 U/µL) | کمپانی Thermo Fisher Scientific
آنزیم Eco47III (AfeI) (10 U/µL) | کمپانی Thermo Fisher Scientific
لطفا جهت مطالعه توضیحات بیشتر این محصول به لینک سایت زیر مراجعه نمایید:
https://www.thermofisher.com/order/catalog/product/ER0322?SID=srch-srp-ER0322
Eco47III (AfeI) (10 U/µL) Catalog number: ER0322 Size: 1,000 units
Eco47III (AfeI) (10 U/µL) Catalog number: ER0321 Size: 200 units
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توضیحات محصول
آنزیم Eco47III (AfeI) (10 U/µL) | کمپانی Thermo Fisher Scientific
Catalog Number: ER0321
Unit Size: 200 Units
Catalog Number: ER0322
Unit Size: 1000 Units
| 5′ | A | G | C ↓ | G | C | T | 3′ | ||||
| 3′ | T | C | G ↑ | C | G | A | 5′ |
Thermo Scientific Eco47III (AfeI) restriction enzyme recognizes AGC^GCT sites and cuts best at 37°C in O buffer. See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Note: Also available as a FastDigest enzyme for rapid DNA digestion. Isoschizomers: AfeI, Aor51HI.
Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that sent in DNA preparations.
Features
• Superior quality—stringent quality control and industry leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
• Wide selection of restriction endonuclease specificities
Applications
• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern blotting
• Restriction fragment length polymorphism (RFLP)
• SNP
Note: For methylation sensitivity, refer to product specifications.
| Specifications | |
|---|---|
| Concentration | 10U/μL |
| Methylation Sensitivity | CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive |
| Enzyme | Eco47III (AfeI) |
| Compatible Buffer | 10X Buffer O |
| Sensitive to Heat Inactivation | Yes |
| Optimal Reaction Temperature | 37°C |
| Type IIS RE | No |
| Quantity | 1000U |
| Product Type | Restriction Enzyme |
| Research Category | Traditional cloning |
بررسی تخصصی
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