آنزیم BglI (10 U/µL) | کمپانی Thermo Fisher Scientific

آنزیم BglI (10 U/µL) | کمپانی Thermo Fisher Scientific

Catalog Number: ER0071

Unit Size: 2000 Units

Catalog Number: ER0072

Unit Size: 5 x 2000 Units

5′

G

C

C

N

N

N

N ↓

N

G

G

C

3′

3′

C

G

G

N ↑

N

N

N

N

C

C

G

5′

Thermo Scientific BglI restriction enzyme recognizes GCCNNNN^NGGC sites and cuts best at 37°C in buffer O. See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Note: Also available as a FastDigest enzyme for rapid DNA digestion.

Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

Features

• Superior quality—stringent quality control and industry leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
• Wide selection of restriction endonuclease specificities

Applications

• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern blotting
• Restriction fragment length polymorphism (RFLP)
• SNP

Note: For methylation sensitivity, refer to product specifications.

	Specifications
10U/μL	Concentration
CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive	Methylation Sensitivity
Bgl I	Enzyme
10X Buffer O	Compatible Buffer
Yes	Sensitive to Heat Inactivation
37°C	Optimal Reaction Temperature
No	Type IIS RE
5x 2000 U	Quantity
Restriction Enzyme	Product Type
Traditional cloning	Research Category

آنزیم BglI (10 U/µL) | کمپانی Thermo Fisher Scientific