کیت استخراج رسوبی DNA از خون ومایعات بدن | Blood and Body Fluids isolation kit-Solution Based

قیمت کیت استخراج رسوبی DNA از خون ومایعات بدن |قیمت Blood and Body Fluids isolation kit-Solution Based

REDNAKIA PLUS

Blood and Body Fluids isolation kit (Solution Based)

Cat. No:

FPKT026.0025

FPKT026.0050

FPKT026.0100

Contents:

Kit storage:

This kit should be stored at room temperature.

If properly stored, all kit components are stable until the expiration date printed on the label.

Additional Equipment and Reagent required

• Absolute ethanol
• Ethanol 70%
• Chloroform
• Standard tabletop microcentrifuge capable of 13,000 x g centrifugal force
• Microcentrifuge tubes, 1.5 ml, sterile

Application

This kit provides a convenient and rapid method to isolate total DNA from fresh, frozen and preserved Blood samples. The purified DNA is of the highest quality and is fully compatible with all downstream applications such as PCR, qPCR, NGS and microarrays.The procedure is optimized to achieve reliable results within 15 min.

Handling Requirements and Safety Information

All solutions are clear and should not be used when precipitates have formed. Warm the solutions at +15 to +25°C or in a 37°C water bath until the precipitates have dissolved.

Do not allow Chloroform to touch your skin, eyes, or mucous membranes. If contact does occur, wash the affected area immediately with large amounts of water.

If you spill the reagent, dilute the spill with water before wiping it up.

Do not use any modified ethanol.

Do not pool reagents from different lot numbers.

Immediately after usage, close all bottles in order to avoid leakage, varying buffer concentrations or buffer conditions.

Wear protective disposable gloves, laboratory coats and eye protection, when handling samples and kit reagents.

Do not contaminate the reagents with bacteria, virus, or nucleases. Use disposable pipets and nuclease free pipet tips only, to remove aliquots from reagent bottles.

preparation procedure:

before starting Incubate the PEB buffer at 55 to 65 ° C until the end of the protocol to obtain the maximum yields.

Protocol

1. Add 1 ml RD1 Buffer to the 2ml microtube and add 500 μl Blood or 800 μl serum, plasma, body fluids or Amniotic Fluid samples. Mix gently (don’t Vortex) and centrifuge at maximum speed for 1 minutes.
2. Discard the supernatant and add 1ml RD1 Buffer again. Mix gently (don’t Vortex) and centrifuge at maximum speed for 1 minutes.
3. Discard the supernatant and Add 300 μl RD2 Buffer. Pipette completely.
4. Add 300 μl RD3 Buffer then add 600 μl Chloroform mix vigorously for 10 seconds (don’t Vortex). Centrifuge at 7000 RPM for 4 min.
5. Transfer the upper solution layer into a 1.5 ml microtube gently. Add 1ml chilled Absolute ethanol to the sample. mix gently (don’t Vortex). Centrifuge at Maximum speed for 2 min at +4°C. (a thin and white pellet should be seen in bottom of microtube).
6. Discard supernatant carefully. Add 1ml chilled 70% Ethanol and invert several. Centrifuge at Maximum speed for 2 min at +4°C.
7. Discard supernatant carefully. to remove residual ethanol, incubate microtube at +55°C on hot plate for 5-10 min.
8. Add 50 μl PEB Buffer or sterile DDW on pellet and pipette gently.
9. Check 5-10 μl of extracted DNA by 1% agarose gel electrophoresis. Store DNA at -20˚C.

کیت استخراج رسوبی DNA از خون ومایعات بدن | Blood and Body Fluids isolation kit-Solution Based

قیمت کیت استخراج رسوبی DNA از خون ومایعات بدن |قیمت Blood and Body Fluids isolation kit-Solution Based