پروتکل و دستورالعمل ساخت بافر تی بی ایی | TBE buffer Recipe protocol

TBE buffer Recipe – TBE buffer protocol

Prepare a 5X stock solution in 1 L of H₂O:

54 g of Tris base

27.5 g of boric acid

20 mL of 0.5 M EDTA (pH 8.0)

The 0.5X working solution is 45 mM Tris-borate/1 mM EDTA.

TBE is usually made and stored as a 5X or 10X stock solution. The pH of the concentrated stock buffer should be ~8.3. Dilute the concentrated stock buffer just before use and make the gel solution and the electrophoresis buffer from the same concentrated stock solution. Some investigators prefer to use more concentrated stock solutions of TBE (10X as opposed to 5X). However, 5X stock solution is more stable because the solutes do not precipitate during storage. Passing the 5X or 10X buffer stocks through a 0.22-μm filter can prevent or delay formation of precipitates.