کیت استخراج پلازمید به روش رسوبی | Plasmid DNA Isolation solution based kit
Eco Kit FPKT020.0025
mini Kit FPKT020.0050
Midi Kit FPKT020.0100
Maxi Kit FPKT020.0200
از: 2,500,000 ریال
کیت استخراج پلازمید به روش رسوبی | Plasmid DNA Isolation solution based kit
This kit should be stored at room temperature.
RNase A should be stored at -20 °C
If properly stored, all kit components are stable until the expiration date printed on the label.
After adding RNaseA to PSB1 buffer Store the PSB1 at +2 to +8°C
Isolation of up to 15μg purified plasmid DNA from bacterial cultures, which may be used directly in downstream applications such as restriction enzyme digestion, PCR, cloning, sequencing, in vitro transcription, or labeling reactions. The procedure is optimized to achieve reliable results within 90 min.
All solutions are clear and should not be used when precipitates have formed. Warm the solutions at +15 to +25°C or in a 37°C water bath until the precipitates have dissolved.
PSB4 contain Phenol which is an irritant and toxic.
Do not allow PSB4 Buffers to touch your skin, eyes, or mucous membranes. If contact does occur, wash the affected area immediately with large amounts of water.
If you spill the reagent, dilute the spill with water before wiping it up.
Do not use any modified ethanol.
Do not pool reagents from different lot numbers.
Immediately after usage, close all bottles in order to avoid leakage, varying buffer concentrations or buffer conditions.
After first opening store all bottles in an upright position.
Do not allow the PSB4 Buffers to mix with sodium hypochlorite found in commercial bleach solutions. This mixture can produce a highly toxic gas.
Wear protective disposable gloves, laboratory coats and eye protection, when handling samples and kit reagents.
Do not contaminate the reagents with bacteria, virus, or nucleases. Use disposable pipets and nuclease free pipet tips only, to remove aliquots from reagent bottles.
Working Solution Preparation
Add RNase A to PSB1 Buffer completely Dissolve the RNase A by vortexing. store the PSB1 buffer at 4 °C.
Pellet the bacterial cells from 1-5 ml of E. coli culture (OD600= 1.5-5 per ml) by centrifuge in 8000g for 1 min. Discard the supernatant (Do not use more highly concentrated sample)
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