KlenTaq DNA polymerase

Cat. No:

FPLF005.0250

FPLF005.0500

Contents:

Description:

KlenTaq DNA Polymerase has no the N-terminal portion of the gene, encoding Thermus aquaticus (Taq) DNA polymerase, leaving a highly active and even more thermal stable DNA polymerase activity.

KlenTaq has a wide range of optimal MgCl2 concentration. The optimal range of Mg2+ concentration for KlenTaq is broader than for the majority of thermostable polymerases. The mutation rate during polymerization is two-fold lower for KlenTaq in comparison with full-length Taq DNA polymerase.

This product is suitable for mutation analysis with mutation-specific oligonucleotides. It has a very low background ability to extend a mismatched 3’-oligonucleotide end making it suitable for mutation analysis with mutation-specific oligonucleotides. Amplicons are T/A cloning compatible.

Kit storage:

This kit should be stored at -20 ^oC. Under this condition reagents are stable for two years from the date of production.

Protocol:

1-Thaw 10X reaction buffer, dNTP mixture.

2-Mix the master mix thoroughly and dispense appropriate volumes into PCR tubes or plates.

3-Add templates DNA to the individual PCR tubes or wells containing the master mix.

4-Program the PCR machine according to the program outlined.

Note:

* Extension temperature is between 68 and 72°C. We highly recommend 68 °C for more efficiency of Klen Taq DNA polymerase..

* For PCR products longer than 3~4 Kb, use an extension time of approximately 1 min per Kb DNA.

Disclaimers and Addresses:

This product is for Research use only and should only be used by trained professionals.

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KlenTaq DNA polymerase is a modified version of the Taq DNA polymerase enzyme used in PCR applications. It is a fusion protein composed of the Klenow fragment of E. coli DNA polymerase I and Taq DNA polymerase. The Klenow fragment has 3′ to 5′ exonuclease activity, which allows for proofreading during DNA synthesis, while Taq DNA polymerase has high processivity and fast extension rate. The combination of these two activities results in a DNA polymerase enzyme that exhibits both high fidelity and robustness in PCR applications.

KlenTaq DNA polymerase is particularly useful for amplifying long or complex DNA templates that require high fidelity during amplification. Its proofreading activity reduces the error rate during DNA synthesis, while its fast extension rate allows for rapid amplification of long DNA fragments. KlenTaq DNA polymerase has been shown to have high specificity, sensitivity, and yield in various PCR applications, such as gene expression analysis, genotyping, and DNA sequencing.

In summary, KlenTaq DNA polymerase is a modified version of Taq DNA polymerase that offers both high fidelity and robustness in PCR applications. Its fusion with the Klenow fragment of E. coli DNA polymerase I allows for proofreading activity during DNA synthesis, while retaining the fast extension rate and high processivity of Taq DNA polymerase. Its use has enabled the development of many molecular biology applications that require accurate and efficient DNA amplification.

Pfu DNA polymerase and KlenTaq DNA polymerase are two different enzymes commonly used in PCR applications. While they are both DNA polymerases, there are some differences between them in terms of their properties and applications.

Pfu DNA polymerase is a high-fidelity DNA polymerase derived from the bacterium Pyrococcus furiosus. It has a proofreading function and a lower error rate compared to Taq DNA polymerase. Pfu DNA polymerase has a slower extension rate compared to Taq DNA polymerase and requires higher annealing temperatures during PCR. It is particularly useful for amplifying long or complex DNA templates that require high fidelity.

KlenTaq DNA polymerase, on the other hand, is a fusion protein composed of the Klenow fragment of E. coli DNA polymerase I and Taq DNA polymerase. It has both proofreading and fast extension rate activities, resulting in an enzyme that exhibits both high fidelity and robustness in PCR applications. KlenTaq DNA polymerase is particularly useful for amplifying long or complex DNA templates that require both high fidelity and fast extension rates.

In summary, the main difference between Pfu DNA polymerase and KlenTaq DNA polymerase is that Pfu DNA polymerase has a higher fidelity and a slower extension rate, while KlenTaq DNA polymerase has both high fidelity and fast extension rates due to its fusion with the Klenow fragment of E. coli DNA polymerase I. The choice between these enzymes depends on the specific PCR application and the requirements for fidelity and extension rates.

Pfu DNA polymerase, KlenTaq DNA polymerase, and Phusion DNA polymerase are three different enzymes used in PCR applications. Each of these enzymes has unique properties that make them suitable for different PCR applications.

Pfu DNA polymerase is a high-fidelity DNA polymerase derived from the bacterium Pyrococcus furiosus. It has a proofreading function and a lower error rate compared to Taq DNA polymerase. Pfu DNA polymerase has a slower extension rate compared to Taq DNA polymerase and requires higher annealing temperatures during PCR. It is particularly useful for amplifying long or complex DNA templates that require high fidelity.

KlenTaq DNA polymerase is a fusion protein composed of the Klenow fragment of E. coli DNA polymerase I and Taq DNA polymerase. It has both proofreading and fast extension rate activities, resulting in an enzyme that exhibits both high fidelity and robustness in PCR applications. KlenTaq DNA polymerase is particularly useful for amplifying long or complex DNA templates that require both high fidelity and fast extension rates.

Phusion DNA polymerase is a high-fidelity DNA polymerase that has both proofreading and fast extension rates. It is a fusion protein composed of the Pyrococcus-like proofreading domain and the Taq-like polymerase domain. Phusion DNA polymerase has a higher fidelity and faster extension rate compared to Pfu DNA polymerase. It is particularly useful for amplifying long or GC-rich DNA templates and for generating blunt-end PCR products.

In summary, the main differences between these three enzymes are their fidelity, extension rates, and annealing temperatures. Pfu DNA polymerase has the highest fidelity but the slowest extension rate, KlenTaq DNA polymerase has both high fidelity and fast extension rates, and Phusion DNA polymerase has high fidelity and fast extension rates, making it suitable for challenging PCR applications. The choice between these enzymes depends on the specific PCR application and the requirements for fidelity, extension rates, and amplification of challenging templates.

آنزیم KlenTaq DNA Polymerase

KlenTaq DNA polymerase نسخه اصلاح شده‌ی آنزیم Taq DNA polymerase است که در برنامه‌های PCR استفاده می‌شود. این پروتئین ترکیبی از قطعه Klenow از پلیمراز DNA I باکتری E. coli و Taq DNA polymerase است. قطعه Klenow فعالیت اگزونوکلئاز 3′ تا 5′ را دارد که در طول سنتز DNA، امکان تصحیح خطاها را فراهم می‌کند، در حالی که Taq DNA polymerase دارای فرایند پردازشی بالا و نرخ تمدید سریع است. ترکیب این دو فعالیت باعث تولید یک آنزیم پلیمراز DNA می‌شود که هم دقت بالا و هم پایداری را در برنامه‌های PCR ارائه می‌دهد.

KlenTaq DNA polymerase به خصوص برای تکثیر الگوهای DNA طولانی یا پیچیده که نیاز به دقت بالا در طول تکثیر دارند، مفید است. فعالیت تصحیح خطا در طول سنتز DNA باعث کاهش نرخ خطا در طول تکثیر می‌شود، در حالی که نرخ تمدید سریع آن امکان تکثیر سریعی از قطعات DNA طولانی را فراهم می‌کند. KlenTaq DNA polymerase در برنامه‌های مختلف PCR، مانند تجزیه و تحلیل بیان ژنی، ژنوتایپینگ و توالی‌یابی دی‌ان‌ای، دقت بالا، حساسیت و بهره وری بالایی را نشان داده است.

به طور خلاصه، KlenTaq DNA polymerase نسخه اصلاح شده‌ی Taq DNA polymerase است که هم دقت بالا و هم پایداری را در برنامه‌های PCR ارائه می‌دهد. ترکیب آن با قطعه Klenow از پلیمراز DNA I باکتری E. coli، فعالیت تصحیح خطا را در طول سنتز DNA فراهم می‌کند، در حالی که نرخ تمدید سریع و فرایند پردازشی بالای Taq DNA polymerase حفظ می‌شوند. استفاده از آن، توسعه بسیاری از برنامه‌های بیولوژی مولکولی را که نیاز به تکثیر دقیق و کارآمد DNA دارند، ممکن کرده است.

KlenTaq شرکت کیاژن فناور آریا:

کیت KlenTaq تولیدی شرکت کیاژن فناور آریا حاوی 1 میلی لیتر محلول MgCl2 و 1 میلی لیتر بافر مورد نیاز آنزیم است و 500 یونیت آنزیم KlenTaq DNA Polymerase می باشد. از این کیت می توان جهت تکثیر قطعه DNA مورد نظر استفاده کرد. برای تکثیر قطعه DNA مورد نظر علاوه بر این کیت به dNTP نیز نیاز خواهید داشت که می توانید آن را جداگانه از طریق لینک خریداری نمایید.