دستورالعمل و پروتکل تهیه استوک آکریل آمید(40% T, 2.67% C) | Acrylamide stock (40% T, 2.67% C) recipe protocol

دستورالعمل و پروتکل تهیه استوک آکریل آمید(40% T, 2.67% C) | Acrylamide stock (40% T, 2.67% C) recipe protocol

38.96 g acrylamide

1.04 g bisacrylamide

Add acrylamide and bisacrylamide to 100 mL of H₂O. Filter the stock solution using Whatman filter paper and store at 4°C. Prepare fresh acrylamide stock solution every few weeks.

T denotes the total percentage concentration of the monomers (acrylamide plus N,N′-methylenebisacrylamide) in grams per 100 mL. C denotes the percentage (by weight) of the cross-linker N,N′-methylenebisacrylamide relative to the total amount of monomers.