WELQut Protease | ول کیوت پروتئاز

WELQut Protease (5 U/µL)

Cat. No:

FPLF016.0250

FPLF016.0500

FPLF016.1000

Contents:

Kit storage:

This kit should be stored at: -20°C short term (4 months), -80°C long term (more than 1 year).

If properly stored, all kit components are stable until the expiration date printed on the label.

WElQut Protease Storage Buffer:

WElQut Protease contains 10 mM Na2HPO4, 2.7 mM KCl, 1.8 mM KH2PO4, pH 7.3, 140 mM NaCl and, 50% glycerol.

Description

WELQut Protease is highly specific,recombinant serine protease of Staphylococcus aureus. It recognizes and precisely cleaves recombinant proteins containing an engineered recognition sequence* W- E- L- Q-X (Trp, Glu, Leu,Gln, X can be any amino acid). The protease cleaves outside the recognition sequence without leaving additional amino acids bound to the target protein.The WELQut Protease is active in a broad temperature (4-30°C) and pH (pH 6.5-9.0) range and does not require specific buffers. In addition, this new protease has several procedural advantages – it is ideal for on-column proteolysis reactions and can be easily removed from reaction mixtures using its built-in His-tag.

Features

• Cleaves outside WELQ recognition sequence, without leaving additional amino acids bound to the target protein.

• Highly specific to cognate recognition site, does not generate non-specific product bands, even after long incubation and using excess of protease.

• Easy to remove from the reaction mixture using built in His-tag.
• Ideal for on-column proteolysis reactions.

Applications

Removal of N-terminal fusion tags from recombinant protein preparations.

Definition of Activity Unit

Each unit is defined as the amount of enzyme required to cleave ≥ 99% of 100 μg of a control protein in 16 h at 20°C. Enzyme activity is assayed in 100 μl 100 mM Tris-HCl (pH 8.0).

Molecular Weight

22 kDa monomer.