تریس بیس | Tris Base

Tris (tris(hydroxymethyl)aminomethane)

Tris (tris(hydroxymethyl)aminomethane) is a commonly used buffer in molecular and cellular biology. Tris is a weak base with a pKa of approximately 8.1, making it particularly useful for buffering solutions in the pH range of 7.0 to 9.0.

In molecular biology, Tris is often used in a variety of applications, including electrophoresis, DNA and RNA extraction, and protein purification. Tris is used as a buffering agent in electrophoresis gels to maintain a constant pH throughout the gel during electrophoresis. Tris is also used in DNA and RNA extraction buffers to maintain the pH of the solution and prevent the degradation of the nucleic acids. In protein purification, Tris can be used as a component of the elution buffer to help release the purified protein from the resin.

In cellular biology, Tris can be used as a component of cell culture media to maintain a constant pH and provide a suitable environment for cell growth. Tris can also be used to prepare isotonic solutions for use in cell culture and to buffer solutions used in microscopy.

Overall, Tris is a versatile and widely used buffer in molecular and cellular biology. Its ability to maintain a constant pH in a variety of solutions makes it an important component in many protocols and assays.