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کیت استخراج DNA,RNAویروسی رش|Roche Viral Nucleic Acid Kit

کیت استخراج DNA,RNAویروسی رش|Roche Viral Nucleic Acid Kitکیت استخراج DNA,RNAویروسی رش|Roche Viral Nucleic Acid Kit
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کیت استخراج DNA,RNAویروسی رش|Roche Viral Nucleic Acid Kit

 

محصول کشور آلمان

Roche Diagnostics GmbH Roche Applied Science 68298 Mannheim Germany

Choose the High Pure Viral Nucleic Acid Kit to efficiently isolate viral DNA and RNA from a broad range of research sample materials, including:

  1. Serum
  2. plasma
  3. whole blood
  4. cell culture supernatant
  5. peripheral blood mononuclear cells (PBMCs)
  6. cerebrospinal fluid (CSF)
  7. tongue scrapings
  8. throat wash samples.

Use a simple, rapid protocol to generate high-quality templates for direct use in PCR, RT-PCR, qPCR, and qRT-PCR applications.

 

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تحویل اکسپرس
پشتیبانی 24 ساعته
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توضیحات محصول

کیت استخراج DNA,RNAویروسی رش|Roche Viral Nucleic Acid Kit

کیت استخراج DNA,RNAویروسی رش|Roche Viral Nucleic Acid Kit

کیت استخراج DNA و RNA ویروسی از طیف وسیع نمونه های کلینیکی و تحقیقاتی

همه کیت های استخراج DNA و RNA ویروسی در قالب یک کیت

استخراج DNA و RNA ویروسی با کیفیت از نمونه های :

  1. استخراج DNA و RNA ویروسی از خون
  2. استخراج DNA و RNA ویروسی از پلاسما
  3. استخراج DNA و RNA ویروسی از سرم
  4. استخراج DNA و RNA ویروسی محیط کشت سلول
  5. استخراج DNA و RNA ویروسی از peripheral blood mononuclear cells (PBMCs)
  6. استخراج DNA و RNA ویروسی از محلول شستشوی حلق
  7. استخراج DNA و RNA ویروسی از ترشحات بزاقی و تراشه های زبان
  8. استخراج DNA و RNA ویروسی از مایع مغزی نخاعی

Choose the High Pure Viral Nucleic Acid Kit to efficiently isolate viral DNA and RNA from a broad range of research sample materials, including:
Serum, plasma, whole blood, cell culture supernatant, peripheral blood mono nuclear cells (PBMCs), cerebrospinal fluid (CSF), tongue scrapings, and throat wash samples.

Use a simple, rapid protocol to generate high-quality templates for direct use in PCR, RT-PCR, qPCR, and qRT-PCR applications.

کیت استخراج DNA,RNAویروسی رش|Roche Viral Nucleic Acid Kit

 Use one versatile kit for diverse applications.

Isolate viral DNA and RNA from a wide variety of sample materials, enabling simultaneous analysis of both virus types.

 Save time with a rapid, easy-to-use protocol.

Prepare multiple PCR/RT-PCR templates in 20 minutes, with only 10 minutes of hands-on time.

 Maximize performance and accuracy in downstream assays.

Obtain highly pure, concentrated (50 μl) nucleic acids that provide high sensitivity, reproducibility, and specificity in real-time PCR and other applications.

Procedure

Protocol for isolating viral nucleic acids from 200 μl serum, plasma, or whole blood.

For the isolation of nucleic acids from whole blood, pre-warm the elution buffer to +70°C.

1-To a nuclease-free 1.5 ml microcentrifuge tube:

  • Add 200 µl serum, plasma, or whole blood.
  • Add 200 µl working solution, freshly prepared (carrier RNA-supplemented Binding Buffer).
  • Add 50 µl Proteinase K solution; mix immediately.
  • Incubate for 10 min at +72°C.

2-Add 100 µl Binding Buffer and mix well.

3-To transfer the sample to a High Pure Filter Tube:
• Insert one High Pure Filter Tube into one Collection Tube.
• Pipet the entire sample into the upper reservoir of the Filter Tube.

4-Insert the entire High Pure Filter Tube assembly into a standard table-top centrifuge.

• Centrifuge 1 min at 8,000 × g.

5-After centrifugation:

  • Remove the Filter Tube from the Collection Tube; discard the flow through and the Collection Tube.
  • Combine the Filter Tube with a new Collection Tube.

6-After combining the Filter Tube with a new Collection Tube:

  • Add 500 µl Inhibitor Removal Buffer to the upper reservoir of the Filter Tube.
  • Centrifuge 1 min at 8,000 × g.

7-After centrifugation:

  • Remove the Filter Tube from the Collection Tube; discard the flow through and the Collection Tube.
  • Combine the Filter Tube with a new Collection Tube.

8-After removal of inhibitors:
• Add 450 µl Wash Buffer to the upper reservoir of the Filter Tube. • Centrifuge 1 min at 8,000 × g and discard the flow through.

9-After the first wash and centrifugation:

  • Remove the Filter Tube from the Collection Tube; discard the flow through and the Collection Tube.
  • Combine the Filter Tube with a new Collection Tube.
  • Add 450 µl Wash Buffer to the upper reservoir of the Filter Tube.
  • Centrifuge 1 min at 8,000 × g and discard the flow through.
  • Leave the Filter Tube-Collection Tube assembly in the centrifuge and spin it for 10 s at maximum speed (approx. 13,000 × g) to remove any residual Wash Buffer. The extra centrifugation time ensures removal of residual Wash Buffer.

10-Discard the Collection Tube and insert the Filter Tube into a nuclease- free, sterile 1.5 ml microcentrifuge tube.

11-To elute the viral nucleic acids:
• Add 50 µl Elution Buffer to the upper reservoir of the Filter Tube. • Centrifuge the tube assembly for 1 min at 8,000 × g.

12-The microcentrifuge tube contains the eluted, purified viral nucleic acids.

Use the eluted nucleic acids directly in PCR (10 – 20 µl DNA eluate) or RT-PCR (3.5 µl viral RNA); or, store the eluted viral RNA at –80°C or the viral DNA at +2 to +8°C or –15 to –25°C for later analysis.

    1. کیت استخراج DNA,RNAویروسی رش|Roche Viral Nucleic Acid Kit

      کیت استخراج DNA,RNAویروسی رش|Roche Viral Nucleic Acid Kit

بررسی تخصصی

کیت استخراج DNA,RNAویروسی رش|Roche Viral Nucleic Acid Kit
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6 دیدگاه برای کیت استخراج DNA,RNAویروسی رش|Roche Viral Nucleic Acid Kit

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