آنزیم XceI (NspI) (10 U/µL) | کمپانی Thermo Fisher Scientific
آنزیم XceI (NspI) (10 U/µL) | کمپانی Thermo Fisher Scientific
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https://www.thermofisher.com/order/catalog/product/ER1472?SID=srch-srp-ER1472
XceI (NspI) (10 U/µL) Catalog number: ER1472 Size: 2,500 units
XceI (NspI) (10 U/µL) Catalog number: ER1471 Size: 500 units
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توضیحات محصول
آنزیم XceI (NspI) (10 U/µL) | کمپانی Thermo Fisher Scientific
Catalog Number: ER1471
Unit Size: 500 Units
Catalog Number: ER1472
Unit Size: 2500 Units
| 5′ | R | C | A | T | G ↓ | Y | 3′ | ||||
| 3′ | Y ↑ | G | T | A | C | R | 5′ |
Thermo Scientific XceI (NspI) restriction enzyme recognizes RCATG^Y sites and cuts best at 37°C in Tango buffer. See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Note: Also available as a FastDigest enzyme for rapid DNA digestion. Isoschizomers: BstNSI, NspI.
Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.
Features
• Superior quality—stringent quality control and industry leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
• Wide selection of restriction endonuclease specificities
Applications
• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern blotting
• Restriction fragment length polymorphism (RFLP)
• SNP
Note: For methylation sensitivity, refer to product specifications.
| Specifications | |
|---|---|
| 10U/μL | Concentration |
| Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive | Methylation Sensitivity |
| XceI (NspI) | Enzyme |
| 10X Buffer Tango | Compatible Buffer |
| Yes | Sensitive to Heat Inactivation |
| 37°C | Optimal Reaction Temperature |
| No | Type IIS RE |
| Restriction Enzyme | Product Type |
| Traditional cloning | Research Category |
بررسی تخصصی
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