آنزیم PfoI (10 U/µL) | کمپانی Thermo Fisher Scientific
آنزیم PfoI (10 U/µL) | کمپانی Thermo Fisher Scientific
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PfoI (10 U/µL) Catalog number: ER1751 Size: 200 units
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توضیحات محصول
آنزیم PfoI (10 U/µL) | کمپانی Thermo Fisher Scientific
Catalog Number: ER1751
Unit Size: 200 Units
5′ | T ↓ | C | C | N | G | G | A | 3′ | ||||
3′ | A | G | G | N | C | C ↑ | T | 5′ |
Thermo Scientific PfoI restriction enzyme recognizes T^CCNGGA sites and cuts best at 37°C in Tango buffer. See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Note: Also available as a FastDigest enzyme for rapid DNA digestion.
Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.
Features
• Superior quality—stringent quality control and industry leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
• Wide selection of restriction endonuclease specificities
Applications
• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern blotting
• Restriction fragment length polymorphism (RFLP)
• SNP
Note: PfoI cleavage is impaired by overlapping dam methylation and blocked by overlapping dcm methylation. To avoid dam or dcm methylation, use a dam-, dcm- strain, such as GM2163. For methylation sensitivity, refer to product specifications.
Specifications | |
---|---|
Concentration | 10U/μL |
Methylation Sensitivity | CpG Methylation-Sensitive, Dam Methylation-Sensitive, Dcm Methylation-Sensitive |
Enzyme | PfoI |
Compatible Buffer | 10X Buffer Tango |
Sensitive to Heat Inactivation | Yes |
Optimal Reaction Temperature | 37°C |
Type IIS RE | No |
Product Type | Restriction Enzyme |
Research Category | Traditional cloning |
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