آنزیم FastDigest NotI | کمپانی Thermo Fisher Scientific

Catalog Number: FD0593

Catalog Number:20 reactions

Catalog Number: FD0594

Catalog Number:50 reactions

Catalog Number: FD0595

Catalog Number:150 reactions

Catalog Number: FD0596

Catalog Number:250 reactions

5′

G

C ↓

G

G

C

C

G

C

3′

3′

C

G

C

C

G

G ↑

C

G

5′

Thermo Scientific FastDigest NotI restriction enzyme recognizes GC^GGCCGC site and cuts best at 37°C in 5–15 minutes using universal FastDigest Buffer. Isoschizomers: CciNI.

Thermo Scientific FastDigest NotI is one of an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers.

The universal buffer allows rapid single-, double-, or multiple DNA digestion within 5–15 minutes eliminating any need for buffer change or subsequent DNA clean-up steps. See Reaction Conditions for FastDigest Enzymes for a table of enzyme activity, heat inactivation and incubation times for this and other FastDigest restriction enzymes. DNA modifying enzymes, such as Klenow Fragment, T4 DNA Ligase, alkaline phosphatases and T4 DNA Polymerase all have 100% activity in FastDigest Buffer. Therefore, enzymes for downstream applications can be directly added to the FastDigest reaction mix.

For additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading of digestion reaction products on gels.

Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects.

Features

• 100% activity of all FastDigest enzymes in the universal buffer
• 100% buffer compatibility with downstream applications
• Complete digestion in 5–15 minutes
• Direct loading on gels
• No star activity
• 176 FastDigest enzymes available

Applications

• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern Blot
• Restriction fragment length polymorphism (RFLP)
• SNP analysis

Note: The FastDigest Green Buffer offers the same high performance in DNA digestion and downstream applications as the colorless FastDigest Buffer. For applications that require product analysis by fluorescence excitation (e.g. concentration measurements in UV light) the colorless FastDigest Buffer is recommended.

For methylation sensitivity, refer to product specifications.

	Specifications
CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive	Methylation Sensitivity
Not I	Enzyme
10X FastDigest Buffer/FastDigest Green Buffer	Compatible Buffer
Yes	Sensitive to Heat Inactivation
37°C	Optimal Reaction Temperature
No	Type IIS RE
20μL 50μL 150μL 250μL	Quantity
Restriction Enzyme	Product Type
Traditional cloning	Research Category
FastDigest	Product Line

آنزیم FastDigest NotI | کمپانی Thermo Fisher Scientific