آنزیم FastDigest NheI | کمپانی Thermo Fisher Scientific

آنزیم FastDigest NheI | کمپانی Thermo Fisher Scientificآنزیم FastDigest NheI | کمپانی Thermo Fisher Scientific
برند:

آنزیم FastDigest NheI | کمپانی Thermo Fisher Scientific

لطفا جهت مطالعه توضیحات بیشتر این محصول به لینک سایت زیر مراجعه نمایید:

https://www.thermofisher.com/order/catalog/product/FD0974?SID=srch-srp-FD0974

FastDigest NheI Catalog number: FD0974 Size: 100 reactions

FastDigest NheI Catalog number: FD0973 Size: 50 reactions

واردات محصول فوق سه هفته الی یک ماه زمان بر خواهد بود

ریال

تحویل اکسپرس
پشتیبانی 24 ساعته
پرداخت در محل
7 روز ضمانت بازگشت
ضمانت اصل بودن کالا

توضیحات محصول

آنزیم FastDigest NheI | کمپانی Thermo Fisher Scientific

آنزیم FastDigest NheI | کمپانی Thermo Fisher Scientific

Catalog Number: FD0973

Catalog Number: 50 reactions

Catalog Number: FD0974

Catalog Number: 50 reactions

5′ G ↓ C T A G C 3′
3′ C G A T C ↑ G 5′


Thermo Scientific FastDigest NheI restriction enzyme recognizes G^CTAGC site and cuts best at 37°C in 5–15 minutes using universal FastDigest Buffer. Isoschizomers: AsuNHI, BmtI.

Thermo Scientific FastDigest NheI is one of an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers.

The universal buffer allows rapid single-, double-, or multiple DNA digestion within 5–15 minutes eliminating any need for buffer change or subsequent DNA clean-up steps. See Reaction Conditions for FastDigest Enzymes for a table of enzyme activity, heat inactivation and incubation times for this and other FastDigest restriction enzymes. DNA modifying enzymes, such as Klenow Fragment, T4 DNA Ligase, alkaline phosphatases and T4 DNA Polymerase all have 100% activity in FastDigest Buffer. Therefore, enzymes for downstream applications can be directly added to the FastDigest reaction mix.

For additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading of digestion reaction products on gels.

Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects.

Features

• 100% activity of all FastDigest enzymes in the universal buffer
• 100% buffer compatibility with downstream applications
• Complete digestion in 5–15 minutes
• Direct loading on gels
• No star activity
• 176 FastDigest enzymes available

Applications

• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern Blot
• Restriction fragment length polymorphism (RFLP)
• SNP analysis

Note: The FastDigest Green Buffer offers the same high performance in DNA digestion and downstream applications as the colorless FastDigest Buffer. For applications that require product analysis by fluorescence excitation (e.g. concentration measurements in UV light) the colorless FastDigest Buffer is recommended.

For methylation sensitivity, refer to product specifications.

Specifications
CpG Methylation-Sensitive,
Not Dam Methylation-Sensitive,
Not Dcm Methylation-Sensitive
Methylation Sensitivity
Nhe I
Enzyme
10X FastDigest Buffer/FastDigest Green Buffer
Compatible Buffer
YesSensitive to Heat Inactivation
37°C
Optimal Reaction Temperature
NoType IIS RE
Restriction Enzyme
Product Type
Traditional cloning
Research Category
FastDigestProduct Line

آنزیم FastDigest NheI | کمپانی Thermo Fisher Scientific

بررسی تخصصی

آنزیم FastDigest NheI | کمپانی Thermo Fisher Scientific
سایر ویژگی‌ها
واکنش(ری اکشن) را انتخاب کنید
100

50

دیدگاه کاربران

38 دیدگاه برای آنزیم FastDigest NheI | کمپانی Thermo Fisher Scientific

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