آنزیم FastDigest FspBI | کمپانی Thermo Fisher Scientific
آنزیم FastDigest FspBI | کمپانی Thermo Fisher Scientific
لطفا جهت مطالعه توضیحات بیشتر این محصول به لینک سایت زیر مراجعه نمایید:
https://www.thermofisher.com/order/catalog/product/FD1764?SID=srch-srp-FD1764
FastDigest FspBI Catalog number: FD1764
واردات محصول فوق سه هفته الی یک ماه زمان بر خواهد بود
1 ریال
هم اکنون در انبار موجود نیست - اما میتوانید این محصول را پیش خرید کنید
توضیحات محصول
آنزیم FastDigest FspBI | کمپانی Thermo Fisher Scientific
Catalog Number: FD1764
| 5′ | C ↓ | T | A | G | 3′ | ||||
| 3′ | G | A | T ↑ | C | 5′ |
Thermo Scientific FastDigest FspBI restriction enzyme recognizes C^TAG site and cuts best at 37°C in 5–15 minutes using universal FastDigest Buffer. Isoschizomers: BfaI, BfaI, MaeI, XspI.
Thermo Scientific FastDigest FspBI is one of an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers.
The universal buffer allows rapid single-, double-, or multiple DNA digestion within 5–15 minutes eliminating any need for buffer change or subsequent DNA clean-up steps. See Reaction Conditions for FastDigest Enzymes for a table of enzyme activity, heat inactivation and incubation times for this and other FastDigest restriction enzymes. DNA modifying enzymes, such as Klenow Fragment, T4 DNA Ligase, alkaline phosphatases and T4 DNA Polymerase all have 100% activity in FastDigest Buffer. Therefore, enzymes for downstream applications can be directly added to the FastDigest reaction mix.
For additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading of digestion reaction products on gels.
Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects.
Features
• 100% activity of all FastDigest enzymes in the universal buffer
• 100% buffer compatibility with downstream applications
• Complete digestion in 5–15 minutes
• Direct loading on gels
• No star activity
• 176 FastDigest enzymes available
Applications
• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern Blot
• Restriction fragment length polymorphism (RFLP)
• SNP analysis
Note: The FastDigest Green Buffer offers the same high performance in DNA digestion and downstream applications as the colorless FastDigest Buffer. For applications that require product analysis by fluorescence excitation (e.g. concentration measurements in UV light) the colorless FastDigest Buffer is recommended.
For methylation sensitivity, refer to product specifications.
| Specifications |
|
|---|---|
| CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive | Methylation Sensitivity |
| FspBI | Enzyme |
| 10X FastDigest Buffer/FastDigest Green Buffer | Compatible Buffer |
| Yes | Sensitive to Heat Inactivation |
| 37°C | Optimal Reaction Temperature |
| No | Type IIS RE |
| Restriction Enzyme | Product Type |
| Traditional cloning | Research Category |
| FastDigest | Product Line |
آنزیم FastDigest FspBI | کمپانی Thermo Fisher Scientific
Leland –
Thiis design is spectacular! Yoou obviously knoow how to keepp
a reazder entertained. Between your wit aand yourr videos, I was
almost moved to start mmy own blogg (well, almost…HaHa!) Great job.
I really lokved wuat youu hhad to say, and more than that, hoow you presented it.
Tooo cool!
Alberto –
Wow, wonderful blog layout! Howw lohg have you been blogging for?
you make blogging look easy. Thee overall look
of your weeb site is excellent, let aloe the content!
Marla –
For the reason thjat tthe admin off this web paghe iss working,
no uncerainty very rapidly iit wjll bee renowned, due too its feature contents.
Star –
Hello I am so grateful I founjd your weblog, I really found youu byy accident, while I
wass searching on Yahoo for something else, Nonetheless I am herde now aand would just like to say cheers forr a remarkable post and a all round interesting blog (I
also love thhe theme/design), I don’t have time to browse it
all at the moment butt I have book-marked itt and also added in your RSS feeds, so wyen I have time I will be back too read a lot
more, Please do keep up the excellentt b.