آنزیم FastDigest DraI | کمپانی Thermo Fisher Scientific

آنزیم FastDigest DraI | کمپانی Thermo Fisher Scientificآنزیم FastDigest DraI | کمپانی Thermo Fisher Scientific
برند:

آنزیم FastDigest DraI | کمپانی Thermo Fisher Scientific

لطفا جهت مطالعه توضیحات بیشتر این محصول به لینک سایت زیر مراجعه نمایید:

https://www.thermofisher.com/order/catalog/product/FD0224?SID=srch-srp-FD0224

FastDigest DraI Catalog number: FD0224 Size: 200 reactions

واردات محصول فوق سه هفته الی یک ماه زمان بر خواهد بود

ریال

هم اکنون در انبار موجود نیست - اما میتوانید این محصول را پیش خرید کنید

تحویل اکسپرس
پشتیبانی 24 ساعته
پرداخت در محل
7 روز ضمانت بازگشت
ضمانت اصل بودن کالا

توضیحات محصول

آنزیم FastDigest DraI | کمپانی Thermo Fisher Scientific

آنزیم FastDigest DraI | کمپانی Thermo Fisher Scientific

Catalog Number: FD0224

Catalog Number: 200 reactions

5′ T T T ↓ A A A 3′
3′ A A A ↑ T T T 5′


Thermo Scientific FastDigest DraI restriction enzyme recognizes TTT^AAA site and cuts best at 37°C in 5–15 minutes using universal FastDigest Buffer.

Thermo Scientific FastDigest DraI is one of an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers.

The universal buffer allows rapid single-, double-, or multiple DNA digestion within 5–15 minutes eliminating any need for buffer change or subsequent DNA clean-up steps. See Reaction Conditions for FastDigest Enzymes for a table of enzyme activity, heat inactivation and incubation times for this and other FastDigest restriction enzymes. DNA modifying enzymes, such as Klenow Fragment, T4 DNA Ligase, alkaline phosphatases and T4 DNA Polymerase all have 100% activity in FastDigest Buffer. Therefore, enzymes for downstream applications can be directly added to the FastDigest reaction mix.

For additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading of digestion reaction products on gels.

Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects.

Features

• 100% activity of all FastDigest enzymes in the universal buffer
• 100% buffer compatibility with downstream applications
• Complete digestion in 5–15 minutes
• Direct loading on gels
• No star activity
• 176 FastDigest enzymes available

Applications

• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern Blot
• Restriction fragment length polymorphism (RFLP)
• SNP analysis

Note: The FastDigest Green Buffer offers the same high performance in DNA digestion and downstream applications as the colorless FastDigest Buffer. For applications that require product analysis by fluorescence excitation (e.g. concentration measurements in UV light) the colorless FastDigest Buffer is recommended.

For methylation sensitivity, refer to product specifications.

Specifications
Not Dam Methylation-Sensitive,
Not Dcm Methylation-Sensitive,
Not CpG Methylation-Sensitive
Methylation Sensitivity
Dra I
Enzyme
10X FastDigest Buffer/FastDigest Green Buffer
Compatible Buffer
Yes
Sensitive to Heat Inactivation
37°C
Optimal Reaction Temperature
No
Type IIS RE
Restriction Enzyme
Product Type
Traditional cloning
Research Category
FastDigestProduct Line

آنزیم FastDigest DraI | کمپانی Thermo Fisher Scientific

بررسی تخصصی

آنزیم FastDigest DraI | کمپانی Thermo Fisher Scientific

دیدگاه کاربران

دیدگاهها

هیچ دیدگاهی برای این محصول نوشته نشده است.

اولین نفری باشید که دیدگاهی را ارسال می کنید برای “آنزیم FastDigest DraI | کمپانی Thermo Fisher Scientific”

نشانی ایمیل شما منتشر نخواهد شد. بخش‌های موردنیاز علامت‌گذاری شده‌اند *