آنزیم دی ان ایز DNase | آنزیم DNase RNase Free

DNase I ، (بدون RNase) یک اندونوکلئاز است که به طور اختصاصی DNA را می شکند تا محصولات دی- ، سه- و الیگونوکلئوتید را با انتهای 5′-فسفریله و 3′-هیدروکسیله آزاد کند. DNase I بر روی DNA تک رشته ای و دو رشته ای ، کروماتین و هیبریدهای RNA: DNA عمل می کند.

DNase I, (RNase-free) is an endonuclease that nonspecifically cleaves DNA to release di-, tri- and oligonucleotide products with 5´-phosphorylated and 3´-hydroxylated ends (1,2). DNase I acts on single- and double-stranded DNA, chromatin and RNA:DNA hybrids.

DNase I (RNase-free) is ideal for:

• Removal of contaminating genomic DNA from RNA samples
• Degradation of DNA templates in transcription reactions

DNase I, RNase Free Solution

1U/μl

Cat. No:

FPLF008.1000

FPLF008.0500

Contents:

Kit storage:

 This kit should be stored at -20 °C.

If properly stored, all kit components are stable until the expiration date printed on the label.

Description

DNase I, RNase-free is an endonuclease that digests single- and double-stranded DNA. It hydrolyzes phosphodiester bonds producing mono- and oligodeoxyribonucleotides with 5′-phosphate and 3′-OH groups.

Application

• Preparation of DNA-free RNA.
• Removal of template DNA following in vitro transcription.
• Preparation of DNA-free RNA prior to RT-PCR and RT-qPCR.
• DNA labeling by nick-translation in conjunction with DNA Polymerase I.
• Studies of DNA-protein interactions by DNase I, RNase-free footprinting.

Handling Requirements and Safety Information

use RNase-free and DNase-free materials

Do not use any modified Protocols.

Do not pool reagents from different lot numbers.

Immediately after usage, close all bottles in order to avoid leakage, varying buffer concentrations or buffer conditions.

After first opening store all bottles in an upright position.

Wear protective disposable gloves, laboratory coats and eye protection, when handling samples and kit reagents.

Do not contaminate the reagents with bacteria, virus, or nucleases. Use disposable pipets and nuclease free pipet tips only, to remove aliquots from reagent bottles.

2. Incubate at 37 °C for 30 min.

3. Add 1 μl 50 mM EDTA and incubate at 65 °C for 10 min. RNA hydrolyzes during heating with divalent cations in the absence of a chelating agent. Alternatively, use phenol/chloroform extraction.