آنزیم AanI (PsiI) (10 U/µL) | کمپانی Thermo Fisher Scientific
آنزیم AanI (PsiI) (10 U/µL) | کمپانی Thermo Fisher Scientific
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AanI (PsiI) (10 U/µL) Catalog number: Er2061 Size: 200 units
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توضیحات محصول
آنزیم AanI (PsiI) (10 U/µL) | کمپانی Thermo Fisher Scientific
Catalog Number: ER2061
Unit Size: 200 Units
5′ | T | T | A ↓ | T | A | A | 3′ | ||||
3′ | A | A | T ↑ | A | T | T | 5′ |
Thermo Scientific AanI (PsiI) restriction enzyme recognizes TTA^TAA sites and cuts best at 37°C in Tango buffer (isoschizomers: PsiI). See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Note: Also available as a FastDigest enzyme for rapid DNA digestion.
Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.
Features
• Superior quality—stringent quality control and industry leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
• Wide selection of restriction endonuclease specificities
Applications
• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern blotting
• Restriction fragment length polymorphism (RFLP)
• SNP
Note: For methylation sensitivity, refer to product specifications.
Specifications | |
---|---|
Concentration | 10U/μL |
Methylation Sensitivity | Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive |
Enzyme | AanI (PsiI) |
Compatible Buffer | 10X Buffer Tango |
Sensitive to Heat Inactivation | Yes |
Optimal Reaction Temperature | 37°C |
Type IIS RE | No |
Product Type | Restriction Enzyme |
Research Category | Traditional cloning |
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