دستورالعمل و پروتکل تهیه استوک آکریل آمید(40% T, 2.67% C) | Acrylamide stock (40% T, 2.67% C) recipe protocol
38.96 g acrylamide
1.04 g bisacrylamide
Add acrylamide and bisacrylamide to 100 mL of H2O. Filter the stock solution using Whatman filter paper and store at 4°C. Prepare fresh acrylamide stock solution every few weeks.
T denotes the total percentage concentration of the monomers (acrylamide plus N,N′-methylenebisacrylamide) in grams per 100 mL. C denotes the percentage (by weight) of the cross-linker N,N′-methylenebisacrylamide relative to the total amount of monomers.
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