کیت استخراج DNA ژنومی رش|Roche genomic DNA isolation kit

کیت استخراج DNA ژنومی از طیف وسیع نمونه های کلینیکی و تحقیقاتی

همه کیت های استخراج DNA ژنومی در قالب یک کیت

استخراج DNA ژنومی با کیفیت از نمونه های :

1. استخراج DNA ژنومی از خون
2. استخراج DNA ژنومی از buffy coat
3. استخراج DNA ژنومی از سلول
4. استخراج DNA ژنومی از خلط
5. استخراج DNA ژنومی از مدفوع
6. استخراج DNA ژنومی از مایعات ریوی
7. استخراج DNA ژنومی از ادرار
8. استخراج DNA ژنومی از نمونه های سوآب
9. استخراج DNA ژنومی از بافت
10. استخراج DNA ژنومی از دم موش
11. استخراج DNA ژنومی از مخمر
12. استخراج DNA ژنومی از بافت پارافینه شده
13. استخراج DNA ژنومی از آب محیطی

Choose the versatile High Pure PCR Template Preparation Kit to rapidly and easily isolate genomic DNA from a wide variety of research sample materials, including: Whole blood, buffy coat, cultured cells, clinical research samples (e.g., sputum, feces, bronchoalveolar lavage [BAL], urine, and swabs), animal tissue, mouse tail, yeast, formalin fixed paraffin-embedded (FFPE) tissue sections, and environmental water samples.
Produce multiple PCR templates in minutes using efficient High Pure spin columns. Generate high-purity DNA that improves reproducibility and reliability in applications such as standard or long template PCR, qPCR, SNP detection, microarray analysis, and Southern blotting.

Maximize flexibility.
Use a single versatile kit to purify DNA from a broad range of sample materials.

Facilitate long template applications.
Efficiently purify high molecular weight DNA (30-50 kb)

Improve assay results.
Ensure optimal performance by efficiently removing contaminants that can interfere with PCR or other downstream applications.

Procedure

Protocol for isolating DNA from 200 μl mammalian blood, buffy coat, or cultured mammalian cells.
Before starting the purification, warm the Elution Buffer to +70°C.
1-To a nuclease-free 1.5 ml microcentrifuge tube:
• Add 200 μl of sample material.
• Add 200 μl Binding Buffer.
• Add 40 μl Proteinase K (reconstituted).
• Mix immediately and incubate at +70°C for 10 min.
2-Add 100 μl Isopropanol and mix well.
3-Insert one High Pure Filter Tube into one Collection Tube.
• Pipet the sample into the upper buffer reservoir of the Filter Tube.
• Insert the entire High Pure Filter Tube assembly into a standard table-top centrifuge.
• Centrifuge 1 min at 8,000 × g.
4-After centrifugation:
• Remove the Filter Tube from the Collection Tube; discard the flow through and the Collection Tube.
• Combine the Filter Tube with a new Collection Tube.
• Add 500 μl Inhibitor Removal Buffer to the upper reservoir of the Filter Tube.
• Centrifuge 1 min at 8,000 × g.
5-Remove the Filter Tube from the Collection Tube; discard the flow through and the Collection Tube.
• Combine the Filter Tube with a new Collection Tube.
• Add 500 μl Wash Buffer to the upper reservoir of the Filter Tube.
• Centrifuge 1 min at 8,000 × g and discard the flow through.
6-Remove the Filter Tube from the Collection Tube; discard the flow through and the Collection Tube.
• Combine the Filter Tube with a new Collection Tube.
• Add 500 μl Wash Buffer to the upper reservoir of the Filter Tube.
• Centrifuge 1 min at 8,000 × g and discard the flow through.
7-After discarding the flow through:
• Centrifuge the entire High Pure assembly for an additional 10 s at full speed. The extra centrifugation time ensures removal of residual Wash Buffer.
• Discard the Collection Tube.
8-To elute the DNA:
• Insert the Filter Tube into a clean, sterile 1.5 ml microcentrifuge tube.
• Add 200 μl prewarmed Elution Buffer to the upper reservoir of the Filter Tube.
• Centrifuge the tube assembly for 1 min at 8,000 × g.
9-The microcentrifuge tube contains the eluted, purified
DNA, which can be used directly or stored at +2 to +8°C or +15 to +25°C for later analysis. For details on adding an optional RNase digestion, see related procedures in the pack insert.

1. 1. کیت استخراج DNA ژنومی رش|Roche genomic DNA isolation kit

      

      کیت استخراج DNA ژنومی رش|Roche genomic DNA isolation kit

      قیمت کیت استخراج DNA ژنومی رش|Roche genomic DNA isolation kit