ای دی تی ای | EDTA

Ethylenediaminetetraacetic acid disodium salt dihydrate

EDTA (ethylenediaminetetraacetic acid) is a versatile chelating agent that is widely used in molecular and cellular biology. It is a synthetic compound that forms a complex with metal ions, such as calcium, magnesium, and iron, and is often used to remove these ions from solution.

In molecular biology, EDTA is commonly used to chelate metal ions that can interfere with enzymatic reactions, such as DNA polymerase activity. EDTA is often included in DNA extraction buffers to remove magnesium ions, which can inhibit the activity of certain enzymes. EDTA can also be used to stabilize proteins and prevent their degradation by metal-dependent proteases.

In cellular biology, EDTA can be used as a chelator to remove calcium ions from the extracellular environment, which can trigger cellular responses such as secretion and contraction. EDTA can also be used to dissociate cells from tissue culture plates, as it can sequester calcium ions and disrupt cell-cell junctions.

Additionally, EDTA can be used to preserve cell morphology in histology and pathology samples. It can chelate calcium ions in tissues, preventing the formation of calcium carbonate deposits that can obscure cellular structures.

Overall, EDTA is a versatile and widely used chelating agent in molecular and cellular biology. Its ability to remove metal ions from solution and stabilize proteins makes it an important component in many protocols and assays.

why is disodium salt of edta preferred to edta?

Ethylenediaminetetraacetic acid disodium salt dihydrate (EDTA-2Na.2H2O) is a derivative of EDTA (ethylenediaminetetraacetic acid) that has two sodium ions and two water molecules associated with it. The main difference between EDTA and EDTA-2Na.2H2O is that EDTA-2Na.2H2O is a salt, while EDTA is an acid.

EDTA-2Na.2H2O is often used in industrial and laboratory applications, as it is more soluble in water than EDTA. EDTA-2Na.2H2O is also commonly used as a chelating agent to sequester metal ions in solution, as it can form stable complexes with a variety of metal ions.

EDTA, on the other hand, is often used in biological applications, such as molecular and cellular biology. EDTA is used to chelate metal ions that can interfere with enzymatic reactions and protein stability. EDTA is also used to remove metal ions from DNA extraction buffers, as they can inhibit the activity of certain enzymes.

Both EDTA and EDTA-2Na.2H2O have similar chelating properties and can be used to sequester metal ions in solution. However, EDTA-2Na.2H2O is more soluble in water and is often used in industrial and laboratory applications, while EDTA is commonly used in biological applications.

Ethylenediaminetetraacetic acid disodium salt dihydrate (EDTA-2Na.2H2O) یک مشتق از EDTA (اسید اتیلن دی آمین تترا استاتیک) است که دو یون سدیم و دو مولکول آب با آن وابسته هستند. اصلی ترین تفاوت بین EDTA و EDTA-2Na.2H2O این است که EDTA-2Na.2H2O یک نمک است در حالی که EDTA یک اسید است.

EDTA-2Na.2H2O در برنامه های صنعتی و آزمایشگاهی به طور گسترده استفاده می شود زیرا بیشتر در آب حل می شود. همچنین EDTA-2Na.2H2O به عنوان یک عامل کلات کننده برای جداسازی یون های فلزی در محلول استفاده می شود زیرا با انواع مختلفی از یون های فلزی، کمپلکس های پایدار تشکیل می دهد.

EDTA به طور گسترده در برنامه های زیست شناسی مولکولی و سلولی استفاده می شود. EDTA برای کلات کردن یون های فلزی که می توانند با واکنش های آنزیمی و پایداری پروتئین تداخل داشته باشند استفاده می شود. همچنین EDTA برای حذف یون های فلزی از بافت های DNA استخراج شده استفاده می شود زیرا می توانند فعالیت برخی از آنزیم ها را مختل کنند.

هر دو EDTA و EDTA-2Na.2H2O ویژگی های کلات کننده مشابهی دارند و می توانند برای جداسازی یون های فلزی در محلول استفاده شوند. با این حال، EDTA-2Na.2H2O در آب بیشتر حل شده و به طور گسترده در برنامه های صنعتی و آزمایشگاهی استفاده می شود.