ROOM-KIARNA-STAB

RNA/DNA Preservation Microtube

Cat. No:

FPST001.0005

FPST001.0025

FPST001.0100

Contents

Kit storage:

 Store ROOM-KIARNA-STAB tubes at room temperature (21–25°C).

Store and transport RNA/DNA samples applied to the tubes at ambient temperature.

Keep tubes sealed and dry.

If properly stored, all kit components are stable until the expiration date printed on the label.

Additional Equipment and Reagents required

• Molecular biology–grade water
• Standard microcentrifuge
• Pipettes and RNase-free tips

Application

ROOM-KIARNA-STAB is a specially formulated RNA/DNA preservation microtube designed to stabilize and protect purified RNA/DNA at room temperature, eliminating the need for dry ice or cold-chain transport. Each microtube contains a dry, water-soluble chemical matrix that inactivates trace RNases and prevents oxidative degradation, allowing safe storage and shipment of RNA/DNA samples in both liquid and dried forms.

ROOM-KIARNA-STAB ensures high RNA/DNA integrity, quantitative recovery, and compatibility with all common downstream applications, including RT-qPCR, gene expression profiling, NGS,and microarray analysis.

Key Features & Advantages

• Immediate RNA/DNA stabilization at room temperature
• Quantitative RNA/DNA recovery equal to the input amount
• Maintains RNA/DNA quality comparable to frozen storage
• Protects against trace RNase and oxidation
• Stability in liquid form for up to 100 hours at room temperature
• Stability after rehydration for up to 8 hours
• Compatible with RNA/DNA from blood, cell lines, tissues, FFPE samples, PAXgene RNA, and all major extraction kits
• No inhibitory effect on RT-qPCR or gene expression analysis
• Thermal stability during transport from −80°C to +76°C
• Store and transport at room temperature—no dry ice required
• Supports up to 5 drying-rehydration cycles

Handling Requirements and Safety Information

Use gloves, lab coat, and eye protection when handling RNA.

Use RNase-free consumables at all stages.

Do not expose tubes to high humidity or moisture.

Avoid RNase contamination during drying. The matrix protects against RNases, but clean handling is recommended.

Protocol

RNA Application

1. Add ≤20 µg RNA/DNA in a final volume of 20–50 µL into the microtube.
   • For concentrated RNA/DNA, add nuclease-free water to reach ≥20 µL.
2. Incubate at room temperature for a few minutes to ensure solubilization.
3. Mix gently by pipetting up and down 10 times.
4. Depending on use, proceed with:
   • Liquid stabilization (short-term use)
   • Drying (long-term storage or shipment)

Apply RNA/DNA according to the protocol above.

Use the stabilized RNA/DNA directly for quantification, Bioanalyzer analysis, RT-qPCR, or other downstream assays.

RNA/DNA remains stable for up to 100 hours at room temperature.

After 100 hours, either dry the sample or store by your standard method.

Dry samples with caps off. Use one of the following methods:

Place uncapped tubes in the chosen drying system.

Dry according to the times above.

After drying, cap tubes and store/ship at ambient temperature.

RNA/DNA Recovery

1. Add molecular-grade water equal to the input volume (e.g., add 20 µL if 20 µL was applied).
2. Incubate at room temperature for 10 minutes.
3. Mix by pipetting up and down 10 times or by gentle vortexing.
4. RNA/DNA is ready for QC and downstream applications.
5. Following recovery, RNA/DNA remains stable for up to 8 hours.
6. For long-term storage, dry again or store using your typical method.